Substrate specificity effects of lipoxygenase products and inhibitors on soybean lipoxygenase-1
نویسندگان
چکیده
منابع مشابه
Studies on the Substrate Specificity of Soybean Lipoxygenase - 1 Using an Entire
Substrate recognition mechanism o f soybean lipoxygenase-1 (L -l) was studied by using an entire series o f (co3Z,co6Z,co9Z)-C12~ C 22-trienoic acids and -trienols. The m axim um activity o f L-l was obtained with C20-trienoic acid o f all the substrates used. A m ong trienols, the m axi mum activity was obtained with C ,5-trienol. Analysis o f the products obtained by the reaction o f these s...
متن کاملLipoxygenase-1 Leaching from Soybean Flour Employing Stirred Tank Contactor
The present work examines leaching of lipoxygenase-1 from soybean flour employing stirred tank vessel. The effect of operating parameters, impellers speed, operational period, temperature, pH and scale-up were considered.The acetic acid pH: 5.2 increased the leaching of lipoxygenase- 1.The sensitivity of agitator speed and geometrical-scale-up on the enzymeleaching has bee...
متن کاملOleyl sulfate reveals allosteric inhibition of soybean lipoxygenase-1 and human 15-lipoxygenase.
Inhibition of lipoxygenase (LO) is currently an important goal of biomedical research due to its critical role in asthma, atherosclerosis, and cancer regulation. Steady-state kinetic data indicate that oleic acid (OA) is a simple competitive inhibitor for soybean lipoxygenase; however, kinetic isotope effect (KIE) data suggest a more complicated inhibitory mechanism. To investigate the inhibito...
متن کاملThe nitric oxide complex of ferrous soybean lipoxygenase-1. Substrate, pH, and ethanol effects on the active-site iron.
Soybean lipoxygenase is a non-heme iron enzyme that catalyzes the hydroperoxidation of linoleic acid by dioxygen. Exposure of ferrous lipoxygenase to nitric oxide yields a species displaying an electron paramagnetic resonance spectrum characteristic of a nearly axial S = 3/2 electronic spin system arising from the ferrous-nitrosyl complex. That spectrum is pH-sensitive, reflecting changes in th...
متن کاملExcess substrate inhibition of soybean lipoxygenase-1 is mainly oxygen-dependent.
Soybean lipoxygenase-1 kinetics are known to show product and substrate inhibition. With linoleic acid as the substrate and using a simple Michaelis-Menten formulation, we have shown that K(ss), the substrate inhibition constant was increased by more than five-fold when initial oxygen concentration was increased from 228 to 1140 microM. Excess substrate inhibition is in fact almost avoided at h...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Bioorganic & Medicinal Chemistry
سال: 2009
ISSN: 0968-0896
DOI: 10.1016/j.bmc.2009.08.005